Biology & Microbiology - Molecular And Microbiology Pace 4 Quiz

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The most abundant genes in prokaryotic genomes are

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The pan genome of a species is the genomic content that is

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An open reading frame (ORF) encodes for

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Microarrays can be used to

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The 140 kbp genome containing many short repeats, ribosomal RNA genes, one RNA polymerase gene (rpo) and one RubisCO gene (rbcL) is most likely from a(n)

6

The evolutionary origin of mitochondria is demonstrated by which of the following key genomic characteristics?

7

Based on the table of average intron frequency given below, predict the relative order of genome size for these four eukaryotic organisms

8

When comparing genes from Archaea, Bacteria, and Eukarya, which statement is TRUE?

9

________ of prokaryotic genomes are now available in public databases

10

Why are microbial doubling times in nature typically longer than those obtained in the laboratory?

11

Justify why clinical medicine has an interest in understanding bacterial cell division at the molecular level

12

Defend and explain how FtsZ-type proteins being found in mitochondria and chloroplasts strengthens or weakens the endosymbiosis theory

13

Propose a growth setup that an industrial microbiologist would create to obtain large quantities of a bacteriocidal product of secondary metabolism

14

Explain our present understanding of molecular adaptations to the cytoplasmic membrane that are present in thermophiles

15

Would you expect a xerophilic organism to be halotolerant? Why or why not?

16

Explain our present understanding of molecular adaptations to the cytoplasmic membrane that are present in psychrophiles

17

Explain why a hyperthermophile is unlikely to be a human pathogen

18

Enumerating the viable and total cell concentration of a population of a microbial isolate can be a really laborious task for a microbiologist

19

Compare and contrast the spread plate method and the pour plate method of doing plate counts. Also describe which group of organisms would not be quantifiable in the pour plate method but would still be observed in the spread plate method

20

Knowing the concentration of microorganisms in a sample is often an important consideration for environmental, industrial, and medical microbiologists, yet a microscope is sometimes not used to accomplish this task

21

Explain the difference between the division rate and the generation time

22

An unpressurized autoclave would not be able to sterilize a growth medium with contaminating bacteria but could still pasteurize it

23

Use the formulas N = N02n and g = t/n. N = final cell number, N0 = initial cell number, n = number of generations, t = time, g = generation time. Find the generation time if N = 2 à— 108, N0 = 3 à— 106 and t = 3 hours

24

A bacterium's pH optimum reflects the optimal pH of its intracellular environment

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The sensitivity of DNA and RNA is one major reason water activity can limit microbial growth

26

Sterility of a laminar flow hood is accomplished by filter-sterilized air passed through the hood quickly enough that non-sterile air does not flow into the work area

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Due to the relationship between the rate of enzyme catalysis and temperature, organisms living in boiling hot springs are also rapid growers with fast doubling times

28

Taq polymerase was isolated from a thermophile and is used in the polymerase chain reaction (PCR) technique because it does not become inactivated at high temperatures

29

In principle, the chemical composition of membranes in obligate acidophiles could be distinguished from neutrophiles

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Biology & Microbiology - Molecular And Microbiology Pace 4 Quiz

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